In-vitro antimicrobial and antioxidant activities of aqueous pericarp extract of Punica granatum.

The study aimed to evaluate the anti microbial against human pathogenic organisms and antioxidant activities of aqueous pericarp extract of Punica granatum. The antimicrobial activity was tested against human pathogenic bacteria and fungi. The aqueous pericarp extract of Punica granatum was showed significant inhibitory activity. 11 mm was the highest inhibitory zone against Staphylococcus aureus but not active against C. albicans. The MIC of pericarp extract was found to be the range between 10- 100 μg/ml. 10 μg/ml was the lowest MIC to most of the bacteria. Radical scavenging activity of pericarp extract of P. granutum was carried out to assess antioxidant activity with employing acorbic acid as standard drug. 6.8, 36.3, 4, 65.2, 10.0, 185 μg/ml were the IC50 values of seed coat extract to Superoxide, Hydroxyl, Hydrogen peroxide, DPPH, Nitric oxide, Lipid peroxide radical scavenging activities respectively. The antimicrobial as well as antioxidant activities were dose dependently increased with pericarp extract concentration. The present study revealed that pericarp of Punica granatum has shown significant antimicrobial and antioxidant activities.

Phylogenetical metabolite logical algorithm: A test using phylogenetic metabolite analysis using TLC in some convolvulaceae members.

Chemical substances act as vital substances occurring in living organisms and can be studied through various biotechnological approaches. The characteristic metabolites of a plant species may reflect evolution towards the resistance produced to environment. Evolvulus alsinoides, Evolvulus nummularius and Merrimia tridentata belongs to convolvulaceae members has been taken as experimental plants in the present study. Thin layer chromatography (TLC) has been used as a data collection technique for identification of similar organisms by logical method, from various Rf values occurred by experimentation. Based on phylogenetic analysis using UPGMA method, E.nummularius has shown good matches with both E.alsinoides and M.tridentata. Hence E.nummularius may be showing evolutionary link between E.alsinoides and M.tridentata. Seventeen compounds are visualized with similar Rf values between all these plant members. Hence most of the data are observed to be having similar compounds, which makes to be placed in same family (convolvulaceae).